Table 2 Gene ontology analysis of Epinephelus coioides larvae

Cellular component

Gene Ontology term

Cluster frequency

Genome frequency of use

Corrected P-value

Myosin complex

45 out of 1129 genes, 4.0%

162 out of 28794 genes, 0.6%

1.46E-23

Actin cytoskeleton

79 out of 1129 genes, 7.0%

656 out of 28794 genes, 2.3%

1.62E-16

Contractile fiber

59 out of 1129 genes, 5.2%

501 out of 28794 genes, 1.7%

1.28E-11

Extracellular region

106 out of 1129 genes, 9.4%

1483 out of 28794 genes, 5.2%

3.55E-07

Myofibril

43 out of 1129 genes, 3.8%

433 out of 28794 genes, 1.5%

6.02E-06

Sarcomere

36 out of 1129 genes, 3.2%

327 out of 28794 genes, 1.1%

6.05E-06

Contractile fiber part

36 out of 1129 genes, 3.2%

332 out of 28794 genes, 1.2%

8.94E-06

Cytoskeleton

173 out of 1129 genes, 15.3%

3012 out of 28794 genes, 10.5%

3.39E-05

Extracellular region part

92 out of 1129 genes, 8.1%

1346 out of 28794 genes, 4.7%

3.53E-05

Striated muscle thin filament

9 out of 1129 genes, 0.8%

25 out of 28794 genes, 0.1%

5.28E-05

Molecular function

Hydrolase activity

283 out of 1138 genes, 24.9%

5158 out of 28380 genes, 18.2%

2.08E-06

Cytoskeletal protein binding

101 out of 1138 genes, 8.9%

1398 out of 28380 genes, 4.9%

2.80E-06

Hydrolase activity, acting on acid anhydrides

132 out of 1138 genes, 11.6%

2114 out of 28380 genes, 7.4%

8.62E-05

Pyrophosphatase activity

131 out of 1138 genes, 11.5%

2097 out of 28380 genes, 7.4%

9.39E-05

Biological process

Lucose metabolic process

42 out of 1105 genes, 3.8%

279 out of 27537 genes, 1.0%

2.39E-10

Hexose metabolic process

48 out of 1105 genes, 4.3%

443 out of 27537 genes, 1.6%

7.72E-07

Muscle system process

52 out of 1105 genes, 4.7%

520 out of 27537 genes, 1.9%

2.54E-06

Small molecule metabolic process

215 out of 1105 genes, 19.5%

3657 out of 27537 genes, 13.3%

4.39E-06

Protein activation cascade

11 out of 1105 genes, 1.0%

31 out of 27537 genes, 0.1%

2.49E-05

Monosaccharide metabolic process

48 out of 1105 genes, 4.3%

493 out of 27537 genes, 1.8%

2.50E-05

Muscle contraction

37 out of 1105 genes, 3.3%

328 out of 27537 genes, 1.2%

2.55E-05

Complement activation

9 out of 1105 genes, 0.8%

20 out of 27537 genes, 0.1%

4.39E-05

Alcohol metabolic process

69 out of 1105 genes, 6.2%

854 out of 27537 genes, 3.1%

4.72E-05