Figure 2

DNA methylation distribution in the bovine gene region. The DNA methylation profile in the gene region was shown by the reads that were aligned on the unique locus in the genome. The gene region was defined as the regions that contained a 2 kb region upstream of the TSS, the gene body from TSS to TTS, and a 2 kb region downstream of the TTS. In the upstream and downstream 2 kb regions, the regions were split into 20 non-overlapping windows, and the average alignment depth was calculated for each window. In the gene body, each gene was split into 40 equal windows, and the average alignment depth was calculated for each window. The y-axis is the average of the normalized depth for each window. The SCNT and control indicated the samples of placental tissues from cloned and normally produced cattle, respectively.