Severe hypoxia exerts parallel and cell-specific regulation of gene expression and alternative splicing in human mesenchymal stem cells

Table 1 HIF-1 regulated transcripts

Gene description	Gene symbol	Fold change HX/NX
Leptin	LEP	74.3
Insulin-like growth factor binding protein 1	IGFBP1	8.93
Phopshoglycerate kinase	PGK	7.10*
Lysyl oxidase-like 4	LOXL4	6.5
Carbonic anhydrase IX	CA9	5.55
Metallothionein 3	MT3	4.78
Vascular Endothelial Growth Factor A	VEGFA	4.61
Carbonic anhydrase XII	CA12	4.62
Lysyl Oxidase	LOX	3.99
Basic helix-loop-helix family member 40	BHLHE40	3.97
Adrenomedullin	ADM	3.39
Placental Growth Factor	PGF	3.28
Phosphofructokinase	PFK	3.20*
Insulin-like growth factor binding protein 3	IGFBP3	3.10
Angiopoietin-like 4	ANGPTL4	2.88
BCL2/adenovirus E1B 19 kDa interacting protein 3	Bnip3	2.17
Lactate Dehydrogenase-A	LDH-A	2.10
Glyceraldehyde-3-Phosphate Dehydrogenase	GAPDH	1.48*
PhopshoGlycerate Mutase	PGAM	0.70*
Solute carrier family 2 (facilitated glucose transporter), member 1	SLC2A1 (GLUT 1)	2.01
Solute carrier family 2 (facilitated glucose transporter), member 3	SLC2A1 (GLUT 3)	2.11

Genes were identified from the gene expression array and all values are significant (p < 0.05, n = 3). For genes with (*), transcripts were quantified by QPCR using 3 different preparations of hMSCs run in duplicate; (PGK p < 0.001; PFK, p < 0.01; GAPDH, p = 0.21; PGAM, p = 0.02; all n = 7).

ISSN: 1471-2164