Table 4 Summary of the toxin-like peptides analysis and comparison with defensin-NV (Ye et al.[51])
CL1Contig4 | CL1Contig1 | CL1Contig5 | Defensin-NV | |
|---|---|---|---|---|
Number of ESTs | ||||
Total | 60 | 33 | 9 | |
FR | 37 | 27 | 6 | |
IT | 23 | 6 | 3 | |
Signal peptide | ||||
TargetP | Yes | Yes | Yes | |
Reliability | Strongest | Strongest | Strongest | |
Sequence length | ||||
Complete (aa) | 60 | 60 | 51 | |
Mature (aa) | 36 | 36 | 27 | |
Molecular weight | ||||
Mature (kDa) | 3.88 | 3.79 | 2.83 | |
Similarity searches | ||||
Swiss prot best hit | U8-theraphotoxin-Cj1a | Conotoxin Vi11.3 | Conotoxin AbVIN | Defensin-1 |
Accession | B1P1C0 | C7DQX8 | Q9TVQ6 | Q5J8R1.1 |
Organism | Chilobrachys jingzhao | Conus vitulinus | Conus abbreviatus | Apis mellifera carnica |
Molecular function | Toxin | Toxin | Toxin | Defensin |
Domain | Knottin | Knottin | Knottin | - |
E-value | 0.026 | 2.9 | 0.34 | 2e-21 |
Toxin prediction | ||||
ClanTox | Toxin-like | Toxin-like | Toxin-like | Toxin-like |
Reliability | Strongest | Strongest | Strongest | Strongest |
Knottin prediction | ||||
Knoter1D | Ambiguous knottin | Ambiguous knottin | Putative knottin | Not a knottin |
AMP Prediction | ||||
AMPer | Antimicrobial peptide Alo-3 | Beta-defensin | Beta-defensin | Defensin |
Lowest HMM E-value | 0.0016 | 0.005 | 0.0054 | 2e-20 |
ClassAMP | Antibacterial | Antibacterial | Antifungal | Antibacterial |
Probability | 0.512 | 0.556 | 0.366 | 0.806 |