Figure 5

Bayesian tree of predicted protein sequences from BLAST-identified ICK toxins of L. hesperus and other spiders. Prefixed identifiers are included for sequences retrieved from the UniProt database. The tree is a midpoint-rooted 50% majority-rule consensus of 3002 trees sampled in Bayesian analysis. Values at nodes are posterior probabilities where they are ≥ 0.95, followed after the slash by ML bootstrap values when >= 70%. Sequences from L. hesperus from this study are in bold and the distinct L. hesperus clade is shaded in red. Red text delineates sequences for which information is available from prior functional studies (see text for details). Tissue expression levels (eCPM) for sequences derived from this study are shown in chart form (Ceph.=cephalothorax, Ven.=venom gland). The two underlined sequences are flipped in position in the ML tree.