Dramatic expansion of the black widow toxin arsenal uncovered by multi-tissue transcriptomics and venom proteomics

Table 3 Summary of putative toxins with no BLAST hit

ID	Clantox	Knoter1D	Iprscan_domains	#Cys	Length
Venom_comp72844_c0_seq1	P3	Knottin	SP, NC	9	83
Contig3061	P2	Knottin	SP, TM, NC, CC	10	98
Contig5795	P3	Knottin	SP, NC	9	95
Contig7277	P2	Knottin	SP, NC	10	91
Venom_comp98528_c0_seq1	P1	Knottin	SP, TM, NC	10	107
Venom_comp75139_c0_seq1	N	Knottin	TM, NC, CP	8	124
Contig20358	N	Knottin	TM, NC, CP	8	116
Venom_Contig10790	P1	Negative	None	6	65
Contig2158	P1	Negative	None	5	52
Venom_Contig4851	P1	Negative	None	5	64

The ID of each sequence is included, together with ClanTox prediction category, Knoter1D prediction of knottin (ICK) scaffold status, protein domains recognized in InterProScan, the number of cysteine residues and protein length. SP = signal peptide, TM = transmembrane domain, NC = non-cytoplasmic domain, CP = cytoplasmic domain, CC = coiled-coil. P3=toxin-like, P2=probably toxin-like, P1=possibly toxin-like, N=probably not toxin-like.

ISSN: 1471-2164