Figure 3

Schematics of the deconvolution algorithm to estimate cell type-specific gene expression. We have measured gene expression by dpp (S), and have estimates of cell type fractions by dpp from the literature (F). Our goal is to estimate gene expression by cell type (C), as well as to re-estimate cell type fractions, or cell type contributions to gene expression (F). The iterative procedure is depicted on the figure, and the details are in Materials and Methods. Due to both biological and mathematical considerations, 5 combined cell types were considered in our analysis.