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Table 3 Mapping features of the two parental linkage maps (Gal1056 and Oria6) and consensus map for the cross (GxO)

From: Genetic control of functional traits related to photosynthesis and water use efficiency in Pinus pinaster Ait. drought response: integration of genome annotation, allele association and QTL detection for candidate gene identification

Mapping features

Gal1056

Oria6

GxO

Total number of available markers

1,539

1,574

2,601

 SSRs loci

8

7

8

 SAMPL loci

29

33

55

 SNP loci

1,502

1,534

2,538

Total number of distorted markersa

33 (2.1%)

36 (2.3%)

53 (2%)

Unlinked markers (%)

65 (4.2%)

78 (5%)

54 (2.1%)

Number of markers assigned to LG

1,474

1,496

2,547

 SSRs loci

8

7

8

 SAMPL loci

21

25

54

 SNP loci

1,445

1,464

2,485

Number of positioned markersb

1,026 (66.7%)

1,184 (75.2%)

1,810 (69.6%)

 SSR loci

2 (25%)

3 (42.9%)

1 (12.5%)

 SAMPL loci

12 (41.4%)

12 (36.4%)

22 (40%)

 SNP loci

1,012 (67.4%)

1,169 (76.2%)

1,787 (70.4%)

Number of positioned genesc

685

792

1,154

Number of distorted positioned markers

0

5

6

LG before alignments

17

16

13

Groups after alignments

12

12

12

Smallest LG before alignments

24 cM

28.7 cM

39.1 cM

Largest LG before alignments

141.9 cM

149.6 cM

165 cM

Average length LG ± SD before alignments (cM)

87.6 ± 42

92.9 ± 41.8

128.9 ± 31.8

Smallest group after alignments

76 cM

70.5 cM

116.1 cM

Largest groups after alignments

187.8 cM

149.6 cM

165 cM

Average length of a group ± SD after alignments (cM)

124.1 ± 26.9

123.9 ± 22.3

138.5 ± 17.1

Maximum distance between 2 adjacent markers

20 cM

28.8 cM

18.3 cM

Average distance between 2 adjacent markers ± SDd

1.92 ± 2.7

1.66 ± 2.6

1.24 ± 1.9

Observed map length (cM)

1,488.7

1,486.8

1,662.3

Estimated map length (cM)

2,337.7

1,479.7

2,378.2

Observed map coverage

64%

100%

69.9%

Estimated map coverage

100%

100%

100%

  1. aAt p < 0.01 after Bonferroni correction for the number of markers.
  2. bNot positioned markers correspond to unlinked markers or markers which position could not be reliably estimated. Percentages calculated over the total number of available markers.
  3. cTwenty one, 47 and 59 positioned contigs for Gal1056, Oria6 and GxO maps respectively, were not considered.
  4. dIdentical markers whose position was the same because of the lack of recombination between them were not considered.
  5. SD: Standard deviation.