Development of highly polymorphic simple sequence repeat markers using genome-wide microsatellite variant analysis in Foxtail millet [Setaria italica (L.) P. Beauv.]

Table 1 Number of polymorphic SSRs among 'Yugu1’, 'Daqingjie’ (DQJ) and 'N10’, and designed primers

Chromosome	Number of SSR sequences	Polymorphic *vs.* DQJ		Polymorphic *vs.* N10		SSR primer design	Number of Polymorphic SSRs	Percentage of polymorphisms
Chromosome	Number of SSR sequences	Number	%	Number	%	SSR primer design	Number of Polymorphic SSRs	Percentage of polymorphisms
Chr.1	607	108	17.8%	215	35.4%	72	67	93.1%
Chr.2	612	121	19.8%	238	38.9%	86	85	98.8%
Chr.3	542	159	29.3%	204	37.6%	90	85	94.4%
Chr.4	396	82	20.7%	157	39.6%	65	62	95.4%
Chr.5	604	158	26.2%	268	44.4%	90	88	97.8%
Chr.6	568	158	27.8%	261	46.0%	123	110	89.4%
Chr.7	372	86	23.1%	173	46.5%	94	86	91.5%
Chr.8	493	153	31.0%	187	37.9%	35	34	97.1%
Chr.9	826	194	23.5%	350	42.4%	133	116	87.2%
Total	5020	1219	24.3%	2053	40.9%	788	733	93.0%

ISSN: 1471-2164