Whole-genome expression profile in zebrafish embryos after chronic exposure to morphine: identification of new genes associated with neuronal function and mu opioid receptor expression

Table 4 Comparison between microarray and RT-qPCR data for selected genes

Transcripts cluster Id	Gene names	Microarray data R.fold	RT‐qPCR data
Transcripts cluster Id	Gene names	Microarray data R.fold	Fold change	P‐value
Up‐regulation
Dr.24320.1.S1_at Dr.21771.1.S1_at	bnip4	2.36/1.23	1.90 ± 0.41*	7.42 E‐05
Dr.9852.1.A1_at	camk1γb	2.16	1.90 ± 0.57*	1.41 E‐05
Dr.7717.2.A1_at Dr.7717.1.A1_at	grb2	2.02/1.58	1.27 ± 0.15*	8.98 E‐04
Dr.3576.1.A1_at	acox1	1.74	2.03 ± 0.43*	2.04 E‐05
Dr.4477.1.A1_at	magi1b	1.30	1.55 ± 0.12*	5.43 E‐05
Dr.3645.1.S1_at	plg	1.26	−1.06 ± 0.21	0.299
*Down‐regulation*
Dr.3663.1.A1_at	dao.1	−1.94	−3.31 ± 0.11*	3.59 E‐08
Dr.25405.1.A1_at	sox19b	−1.80	−2.09 ± 0.19*	0.001
Dr.14687.1.A1_at	copb2	−1.39	−1.69 ± 0.19*	0.004
Dr.3546.1.S1_at	wls	−1.25	−1.67 ± 0.16*	0.006
Dr.3211.1.A1_at	grik‐l	−1.23	−2.94 ± 0.21*	2.53 E‐04
Dr.8118.1.A1_at	otpb	−1.22	−1.64 ± 0.23*	0.004

Values of fold change by RT-qPCR are given as mean of fold change ± standard deviation. The study includes samples used for microarray hybridization and samples from independent treatments; a total of n = 8. For better comparison of the results obtained with both techniques, microarray and RT-qPCR, Morphine vs. Control fold changes are indicated with positive and negative values for up- and down-regulations, respectively. A one-way t-test was performed to determine whether fold changes obtained for morphine-regulated genes were different from 1 and significant values are indicated with asterisks.

ISSN: 1471-2164