Transcriptional and epigenetic responses to mating and aging in Drosophila melanogaster

BMC Genomics

Table 3 Real time PCR confirmation of interactions between miRNAs and target genes*

miRNA	Target gene	Expression in virgin vs. mated female flies ( P-Value)
miRNA	Target gene	Control cross	miRNA overexpression cross	Effect on target gene expression
mir-286	CG15212	0.498	0.037	+
	CG4000	0.033	0.064	+
	dyl	0.241	0.018	+
	Osi7	0.380	0.006	+
	Pkcdelta	0.044	0.364	+
	Vha68-1	0.013	0.348	+
mir-34	CG16857	0.034	0.434	+
	CG34040	0.046	0.036	-
	dan	0.204	0.367	-
	Gbeta76c	0.277	0.010	+
	prc	0.240	0.410	-
	prm	0.060	0.441	-
mir-92b	CG31869	0.351	0.008	+
	CG8303	0.446	0.026	+
	fln	0.378	0.034	+
	Tusp	0.075	0.032	+
mir-988	CG15571	0.038	0.226	+
	CG31086	0.036	0.417	+
	Ela	0.240	0.139	-
	vkg	0.143	0.058	-

*Student’s t-tests were used to calculate P-values of significant differences between expression in virgin and mated flies. Overexpression of a miRNA has an effect on the target gene when differential expression of the target gene is only significant (P <0.05) in control females, but not in the miRNA overexpressing females, or vice versa.

ISSN: 1471-2164