Figure 1

Principal component analysis (PCA) and Venn diagrams of significantly regulated placental transcripts. (A) PCA of gene expression data from DNA microarrays. Each circle represents one analyzed placenta (n = 16) projected on the first three principal components (PC): empty circles, control group (Con); filled circles, n-3 LCPUFA intervention group (N3); red circles, female offspring; blue circles, male offspring. Lines connect outer values of the respective placenta groups: green stippled, control placentas; amber, all N3 placentas; red and stippled red, female placentas of N3 and Con, respectively; blue and stippled blue, male placentas of N3 and Con, respectively. Variability of data points for all female and male placentas is represented by red and blue areas, respectively, enclosing corresponding outer values. (B,C) Shown Venn diagrams compare the number and intersections of significantly regulated transcripts (+1.5 ≤ FC ≤ −1.5, P < 0.05) identified by DNA microarray analysis for the various data sets. (B) Green and amber colored Venn diagrams represent the number of significantly regulated transcripts between male and female placentas in Con [(Con-M; n = 3) vs. (Con-F; n = 4)] and N3 [(N3-M; n = 5) vs. (N3-F; n = 5)], respectively. (C) Red and blue colored Venn diagrams show the number of significantly regulated transcripts between N3 and Con for male [(N3-M; n = 5) vs. (Con-M; n = 3)] and female placentas [(N3-F; n = 4) vs. (Con-F; n =4)], respectively, and for male and female placentas together and adjusted for offspring sex [(N3-M + F; n = 9) vs. (Con-M + F; n = 7)]. White symbols, Con; black symbols, N3; Con-F, female placentas from Con; Con-M, male placentas from Con; N3-F, female placentas from N3; N3-M, male placentas from N3; arrows up or down depict higher or lower expression related to the respective group.