Figure 3

Mapping of sex-determination locus on linkage group 1. Previous studies identifying sex-linked markers on LG1. (a) Lee et al., [24] used a bulked segregant analysis. The green rectangle surrounds markers that were significantly sex-associated. The red rectangle encompasses the region with the highest significance. (b) Ezaz et al., [26] identified three Y-specific AFLPs. OniY425 was assigned through BLAST to scaffold UNK43. It was placed on LG1 according to Lee et al., [25], which used BAC contigs to place it within 100 kb of UNH995. (c) Cnaani et al., [21], also used a bulked segregant analysis. The markers within the red rectangles indicate markers that were significantly associated with sex. (d) Palaiokostas et al., 2013, identified sex-linked RAD-Seq markers. The green rectangle encompasses the markers with a LOD score greater than 15, while the red rectangle encloses the markers flanking the marker with the highest LOD score (Oni23063 with a LOD score of 18.5). (e) Proposed inversion in green with the eight candidate genes discussed in this paper.