Figure 3

Lichenicidin-like lanthipeptides. A Lichenicidin biosynthetic gene cluster (lic) of B. licheniformis in comparison to putative lichenicidin gene clusters of C. botulinum H04402 065 and C. cellulovorans 743B; Numbers represent the locus tag for each gene within the genome sequence of each organism. B Comparison of lichenicidin peptide precursors (LicA1 and LicA2) and the putative precursor peptides of C. botulinum H04402 065 (H04402_00614 and H04402_00615) and C. cellulovorans 743B (Clocel_4229 and Clocel_4226); Glycine-Glycine motif indicates the cleavage site of leader sequence and core peptide (bold) C Formation of lanthionine (lan) and methyl-lanthionine (me-lan) moieties by dehydration of serine/threonine residues to dehydroalanine (dha) / dehydrobutyrine (dhb) and subsequent cyclization with a cysteine residue catalyzed by LanM D Amino acid structure of lichenicidin α-subunit (Bliα).