Table 1 Summary of exome sequencing results of three isogenic HCEC cell lines.
Parameter | 1CT | 1CT7 | A1309 |
|---|---|---|---|
Total reads | 40157067 | 40491020 | 33924594 |
Total yield (bp) | 2066960672 | 2078084457 | 1412998222 |
Mappable reads | 33533312 | 33767043 | 25863504 |
Mappable yield (bp) | 2514998400 | 2532528225 | 1939762800 |
On-target reads | 14514230 | 13119148 | 8353753 |
On-target yield (bp) | 37262779 | 37262779 | 37262779 |
Coverage of target region (more than 10x) | 66.18% | 65.34% | 42.24% |
Mean read depth of targeted region | 20.99 | 19.06 | 10.79 |
Mean read depth of called variants | 23.4 | 23.1 | 21.7 |
Number of total variants | 14466 | 13791 | 6491 |
Number of coding variants | 9841 | 9723 | 4990 |
Number of missense, nonsense, splice, and indel variants | 4910 | 4895 | 2583 |
After NHLBI ESP6500 Exomes filtering | 4071 | 3990 | 2050 |