Figure 2

Confirmation of microarray results. To validate the microarray results seven differentially expressed genes between LN229 and U251 cell lines were selected across the array. (A) Four differentially expressed genes detected using both the amplification and the regular protocols were confirmed by immunochemistry assay (GFAP and S100, positive cells are stained brown) and northern blotting (HLA-DR and SNRP-B). (B) Three other differentially expressed genes, IGFBP2 and integrin beta 4 detected only using the amplification protocol were confirmed by immunohistochemistry assay (IGFBP2), western blotting (IGFBP2) and northern blotting (integrin beta 4).