Figure 5From: Whole-genome microarrays of fission yeast: characteristics, accuracy, reproducibility, and processing of array dataLinear readout range and detection limit of spiked RNA samples. 20 μg of S. pombe total RNA was spiked with five Bacillus subtilis mRNAs at various concentrations. Left side: lysA (3 pg), pheB (15 pg), thrB (30 pg), dapB (150 pg), and trpC (300 pg); right side: lysA (60 pg), pheB (300 pg), thrB (600 pg), and dapB (3000 pg). The median fluorescence intensities above local background of the B. subtilis control spots (determined from ~100 spots/transcript distributed evenly across the array) were plotted as a function of transcript concentration.Back to article page