Figure 1

Multiple Sequence Alignment of the HNOB domain. The multiple sequence alignment was constructed using T-Coffee after parsing high-scoring pairs from PSI-BLAST search results. The PHD-secondary structure is shown above the alignment with E representing a β strand, and H an α-helix (uppercase is for predictions with >82% accuracy while lower case denotes predictions with >72% accuracy). The 90% consensus shown below the alignment was derived using the following amino acid classes: hydrophobic (h: ALICVMYFW, yellow shading); the aliphatic subset of the hydrophobic class are (l; ALIVMC, yellow shading); aromatic (a: FHWY, yellow shading); small (s: ACDGNPSTV, green) and the tiny subset of the small class are (u, GAS, green shading); and polar (p: CDEHKNQRST, blue) with the negative subset (-: DE, pink). A 'G', 'Y' or 'P' shows the completely conserved amino acid in that group. The conserved heme-binding histidine is marked with an asterisk below the consensus. The limits of the domains are indicated by the residue positions on each side. The numbers within the alignment are non-conserved inserts that have not been shown. The sequences are denoted by their gene name followed by the species abbreviation and GeneBank Identifier. The species abbreviations are: Ana – Anabaena Sp; Ccr – Caulobacter crescentus; Cac – Clostridium acetobutylicum; Dde – Desulfovibrio desulfuricans; Mcsp – Magnetococcus sp.; Mde – Microbulbifer degradans; Npu – Nostoc punctiforme; Rhsp – Rhodobacter sphaeroides; Sone – Shewanella oneidensis; Tte – Thermoanaerobacter tengcongensis; Vch – Vibrio cholerae; Ce – Caenorhabditis elegans; Dm – Drosophila melanogaster; Hpul – Hemicentrotus pulcherrimus; Hs – Homo sapiens. The different groups are denoted as: A – Bacterial solos; B – related to solo+X; C – Methyl accepting chemotaxis receptors; D – Guanylyl cyclases; and E – alpha subunits of guanylyl cyclase.