Figure 4

Normalized expression ratio (log₂(Norm/G), where Norm is the total intensity normalization factor) versus average intensity in the red (R) and green (G) channel (1/2(log₂G+log₂R)) plotted on a double logarithmic scale. ScanArray4000 I was used during scanning. The PMT voltage is indicated for the red and green channel separately (% of maximum voltage). The same data set without and with correction of saturated intensities are shown in (A) and (B), respectively. (C), (E), (G), and (I) The intensities in the green channel were above 200 (PMT voltage of 62%), whereas those in the red channel were to an increasing extent below this level (PMT voltages ranging from 56% to 40%). (D), (F), (H), and (J) The intensities in the red channel were above 200 (PMT voltage of 62%), whereas those in the green channel were to an increasing extent below this level (PMT voltages ranging from 56% to 40%). The discontinuity in the distributions at the lowest voltages was due to the apparent discrete values of the lowest intensities and standard deviations, showing values of 1, 2, 3, etc., which occurred because integers were used when assigning the values.