Improving signal intensities for genes with low-expression on oligonucleotide microarrays

BMC Genomics

Table 2 The effect of oligonucleotide length and concentration on average signal intensity ratios.

Experimental Condition		Average Intensity Ratio
		Low-expressing genes*	High-expressing genes*
Oligonucleotide Length @ 50 μM	70	22.1 ± 13.6	7.4 ± 5.4
	50	6.1 ± 3.9	2.7 ± 1.2
	40	2.4 ± 1.3	2.0 ± 0.8
Oligonucleotide Concentration (μM) for length 70	50	3.1 ± 1.8	1.2 ± 0.2
	40	1.9 ± 1.1	0.9 ± 0.2
	30	1.5 ± 0.6	1.1 ± 0.1

* Low- and High-expressing genes are categorized based on relative expression in RKO cell line as shown in Table 1 and definitions are in results and discussion.

ISSN: 1471-2164