Figure 3

A: Fluorescein images of 18,000 element rat cDNA arrays on in-house poly-L-lysine coated slide after printing (A1) and array after non-aqueous post-processing (A2). Fluorescein images of simultaneously 18,000 element rat cDNA arrays on Full Moon Biosystems coated slide (undisclosed chemistry) after printing (A3) and array after non-aqueous post-processing (A4). Note differences in amount of DNA deposited and retained. (White spots are saturated). B: Comparison of retention capacity of 14 different coating surfaces using human 9,600 probe cDNA arrays. Tabulated measurements are based upon 5 replicates slides (~48,000 elements) for each slide type evenly distributed over the arrayer deck (ie 5 slides of a given type did not occupy 5 adjacent positions on the arrayer deck). Slides 1–4 are poly-L-lysine: MCW in-house, Electron Microscopy Sciences, Polysciences, and Cel Associates, respectively. Slides 5–13 are aminated: Asper Biotech, Apogent, Bioslide, Erie Scientific, Genetix, Corning Ultra GAPS, Corning GAPS II, Sigma, and Telechem Super Amine, respectively. Slide 14 is an undisclosed chemistry offered by Full Moon Biosystems. The graph represents the average spot fluorescein intensity RFU/pixel (burgundy) +/- standard deviation (yellow).