Table 1 Comparison of three techniques for AFP identification.
MERITS | FISH ANALYSIS | SOUTHERN ANALYSIS | BAC END SEQUENCING |
|---|---|---|---|
Chromosomal localization | Genomic position | No | Genomic position |
Verification of AFP to original BAC | No | Yes | Yes |
Representation of original BAC in AFP | No | Yes | No |
Relative cost per assay | Expensive | Moderately expensive | Inexpensive |
Time | 3+ Days | 3 days | 4 hours |
Other | Requires normal cell line for metaphase | Requires 200 ng of digested BAC DNA | Can be automated for high throughput analysis |