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Table 6 Performance of a dye combination in revealing modulated genes in B[a]P treated liver slices using an oligonucleotide array.

From: Application of four dyes in gene expression analyses by microarrays

B[a]P concentration

dye combination

3 μM

10 μM

30 μM

Cy5 – Cy3

36*

11

    

Cy3 – A594

64

     

Cy3 – Cy5

  

47

13

  

A594 – Cy3

  

48

   

Cy5 – A594

    

51

8

A594 – Cy5

    

38

 
  1. * In bold the intersection of two gene lists indicating the modulated genes for a dye combination as percentage of all modulated genes (for 3, 10 and 30 μM: 121, 97 and 195 genes respectively) found by analysis of all dye combinations combined. In italics the intersection of three gene lists indicating the modulated genes for two dye combinations as percentage of all modulated genes found for all dye combinations combined. The first dye was used for B[a]P treatment, the second for the control.
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BMC Genomics

ISSN: 1471-2164

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