Table 5 Primers used to amplify short fragments of the Squilla mantis genome. Majority/minority strand are with reference to the ancestral pancrustacean sequence. These primers will work for almost all arthropods and for many other metazoans as well. Reference "JB" is J. Boore pers. comm. Reference "CEC" refers to primers designed for this project.
From: The complete mitochondrial genome of the stomatopod crustacean Squilla mantis
Gene | Majority strand | Sequence | Minority strand | Sequence | Reference |
|---|---|---|---|---|---|
COX1 | Lco1490 | GGTCAACAAATCATAAAGATATTGG | Hco2198 | TAAACTTCAGGGTGACCAAAAAATCA | 38 |
COX1-COX2 | CO1DL | CCWCGWCGWTAYTCWGAYTAYCCWGA | CO2DL | CWGAATARRCATAWSWTCARTATCATTG | JB |
ATP6-COX3 | ATP6.AANMM | GCCGTACGGCTTGCAGCNAAYATRAT | CO3DL2 | ACWACGTCKACGAAGTGTCARTATCA | CEC, JB |
COX3 | CO3DL1 | TGGTGGCGAGATGTKKTNCGNGA | CO3DL2 | ACWACGTCKACGAAGTGTCARTATCA | JB |
ND5 | ND5-R-DL | TARAAKCCWGMTARAAAWGGKAWWCC | ND5-F-DL | TWYTATTAGGKTGAGATGGKYTNGG | JB |
ND4 | ND4-R_DL | GARGAWCAKAWWCCRTGAGCAATYAT | ND4-F-DL | CCKAARGCYCAYGTKGARGCYCC | JB |
CYTB | Cytb424-449 | GGWTAYGTWYTWCCWTGRGGWCARAT | Cytb876-847 | GCRTAWGCRAAWARRAARTAYCAYTCWGG | JB |
ND1 | ND1DL1 | CCTTCWGCAAAATCGAAAGGGGYHCG | ND1.YIQIR | AAGATCCTTGGATAYATYCARATYCG | JB, CEC |
ssrRNA | 12Sb-3' | GAGGGTGACGGGCGGTGTGT | 12sa-5' | AAACTGGGATTAGATACCCTATTAT | JB |
lsrRNA | 16SBRH | CCGGTCTGAACTCAGATCACGT | 16SARL | CGCCTGTTTATCAAAAACAT | 39 |