Table 2 Oligonucleotides used in this study

Psg17A5'

5'-CTTGCCACACAGCCCGTCAT-3'

Psg17 A domain

Psg17A3'

5'-TCATCACAGCCAGGATGACT-3'

Psg17 A domain

mPsg-5'

5'-AWCCTSYTGSYTCCTGC-3'^a

N1 domain

binds to several mouse Psg cDNA sequences

mPsg-3'

5'-TGMARGWAYAKGGATGT-3'^a

N1 domain

binds to several mouse Psg cDNA sequences

PsgN1-F

5'-GAAGATCT AGCCTCCMTYTTDDCCT-3'^a Bgl II

intron 1/N1 exon

for the amplification of all known Psg N1 exons (except Psg32)

PsgN1-R

5'-CCATCGAT TACTTACWGTWSACVTRVA-3'^a Cla I

N1 exon/intron 2

for the amplification of all known Psg N1 exons (except Psg32)

Psg32N1-F

5'-GAAGATCT AGCTTTTCTTTTAACCTC-3' Bgl II

N1 domain

Psg32-exon1

5'-GAGGTGTCCTTGGTGCTTCTC-3'

exon 1

Psg32-specific

oligo (dT)

5'-TTCTAGAATTCAGCGGCCGC(T)₃₀ VN-3'^a

poly(A) tail

Psg-all

5'-CCTCCMTYTTDDCCTRCTGS-3'^a

N1 domain

binds to all known Psg cDNA sequences except Psg32

bCEAN/2

5'-GCAAATGTACAGTGGTAG-3'

N1 domain

Psg16-specific

Psg17N

5'-GTGGAATTCTTACCTCCC-3'

N1 domain

Psg17-specific

Psg18N

5'-GGCTGTACTACTATAGTG-3'

N1 domain

Psg18-specific

BK07

5'-AAAGTGCCACCCGGGAA-3'

N1 domain

Psg19-specific

Psg20N

5'-TGCCAAGGTCACTATCCA-3'

N1 domain

Psg20-specific

Psg21N

5'-GCTCTGCATTTTCTGGAC-3'

N1 domain

Psg21-specific

35N

5'-GTCTGGTATAGAGGGGTG-3'

N1 domain

Psg22-specific

53N

5'-GCTGTGTATTTACTGGAC-3'

N1 domain

Psg23-specific

9.3N1

5'-ATAGCAGAGGTGTGACG-3'

N1 domain

Psg24-specific

11.2N1

5'-ATCTTCTAGGCCTTGCC-3'

N1 domain

Psg25-specific

189N

5'-CATTCGCTGTACTATAGTG-3'

N1 domain

Psg26-specific

214N

5'-CGAGTCACCATCCATTCA-3'

N1 domain

Psg27-specific

2128N

5'-GCACTATAGTTTAACAGCG-3'

N1 domain

Psg28-specific

9140N

5'-TGCAGTGGTGTCTGACTT-3'

N1 domain

Psg29-specific

Psg-ps1N

5'-TTAGTGCCACCACAAGTG-3'

N1 domain

Psg32-specific