Figure 1

Drosophila let-7 miRNA targets the abrupt 3' UTR. A. Predicted sites of let-7 interaction with the ab 3' UTR. The schematic at top shows the relative positions of the sites as vertical bars in the ab 3' UTR. The predicted pairings are shown below, with the free energies (in kcal/mol) and exact positions in the ab 3' UTR indicated. B. Luciferase expression in transfected S2 cells. Expression from the luciferase/ab mRNA with no added miRNA is shown at left, set to a relative value of 1. The other bars indicate the results of coexpression with let-7 or miR-92b. In this figure and in Figure 2, all values represent the average luciferase expression from at least 5 experiments, and error bars represent standard deviation.