Table 2 Forward and reverse primers used to amplify the following genes in Q-RT-PCR assays. House keeping genes are indicated with (*).

von Willebrand factor

For: CTG CCA AGA GGC GAA TAT GT Rev: GCA CAT GTT CTG GTC CCT CT

Phytanoyl-CoA hydroxylase

For: GCC AGC GCA CAC TGT TAT TA Rev: TTT CCT TGC ACA AGA CGT TG

Medium chain S-acyl fatty acid synthetase thio ester hydrolase

For: GAT AAA ACA CTG GGG AGG AAC Rev: CTT GTT ACT TGA CTC CAA CCT TC

Copper/zinc superoxide dismutase (SOD)

For: CTC AGG AGG GTG ATG GAA AA Rev: TTG CCG AAG GGA TTA AAG TG

Activating transcription factor 4 (ATF4)

For: GCG TCC AAG AGA GAA ACA CC Rev: CGA TAT CTT GTG GCA GCA TC

Sphingosine 1-phosphate phosphatase 2

For: TTG TGG CAC GCA CTC TTT AC Rev: TCC CAT TGG TAT CGC TTT TC

Betaine homocysteine methyl transferase

For: ACC AGG TGG GAT ATG CAA AA Rev: CCC CAC ATT TCA TGC TTT TC

Beta-actin *

For: CTG ATG GAC AGG TCA TCA CCA T Rev: CTC GTG GAT ACC AGC AGA TTC C

Putative senescence-associated protein *

For: TCG CAC CAT TGG CTT TTC Rev: CCT ATC ATT GCG AAG CAG AA

Ribosomal protein L12 mRNA *

For: CCT AAG TTC GAC CCC AAT GA Rev: TTT TGG ATG GTC AGT TGG AC