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Figure 1 | BMC Genomics

Figure 1

From: In silico and in vivo splicing analysis of MLH1 and MSH2 missense mutations shows exon- and tissue-specific effects

Figure 1

Schematic representation of the reporter construct used in the splicing assay. Structure of the chimeric minigene used in all the transfection experiments. Patterned and white boxes indicate the pSPL3 HIV-tat exonic sequences and the human hMLH1/hMSH2 sequences. Arrows show the primers used in the RT-PCR experiments. The indicated XhoI and BamHI sites are those used to clone all the constructs. MCS = multi cloning site, Amp = ampicillin resistance gene, M = mutated construct, Wt = corresponding normal exon.

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BMC Genomics

ISSN: 1471-2164

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