Figure 3

GFP-LmjF19.0120 and GFP-LmjF29.0070 localise to the endo-lysosomal pathway in L. major. (A) Co-localisation of GFP-fused LmjF19.0120 with fluorescent endo-lysosomal tracer FM4-64 after incubation for increasing amounts of time (top) with live L. major promastigotes. (B) Localisation of GFP-LmjF19.0120 (top) and GFP-LmjF29.0070 (bottom) in a tubular compartment in live L. major stationary-phase promastigotes. Differential interference contrast (DIC) image, GFP fluorescence image (green) and the signal given by FM4-64 (red) after 30 minutes of incubation are presented in the insets and the two fluorescent signals are shown merged together with the DAPI staining of DNA (blue; n: nucleus; k: kinetoplast, mitochondrial DNA) in the enlarged images. Co-localised signal is shown in yellow. Scale bar = 10 μm.