Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat

Crismani, Wayne; Baumann, Ute; Sutton, Tim; Shirley, Neil; Webster, Tracie; Spangenberg, German; Langridge, Peter; Able, Jason A

doi:10.1186/1471-2164-7-267

BMC Genomics

Table 2 Primer sets used in Q-PCR analysis.

From: Microarray expression analysis of meiosis and microsporogenesis in hexaploid bread wheat

Candidate	Affymetrix probe set	Acquisition temperature	Sense	Antisense	Product size (bp)
			Sequence	Sequence
ASY1	Ta.9186.1.S1_at	83	AGGACTCCCACAAGCAATCG	ACCTGCTGGAGGATCGGCTC	159
DMC1	Ta.30833.1.S1_at	80	GCTGAGGAAAGGCAAAGGCG	CGGTGCAGTAGCCTTTGTCGATTT	195
MSH4	Ta.25861.1.A1_at	83	CTGAAGGATGGTGTCCGACG	AGGTCCTGCAATGCTTCACG	252
MSH6	Ta.24096.1.S1_at	80	CATAATATTGGCACAGATTGGAG	CTGACGAAAGCACGGAAGC	161
PHS1	TaAffx.47591.1.S1_at	80	TGATGCTGCTGGTGGAAATTCG	CGGACACTAGGCATGATAGGCG	270
RAD51B	Ta.7197.1.A1_at	81	CTGCCTGGCTGAAGCTGAAG	GATAGAACAAGCAAACTATGGGAG	267
RAD51C	Ta.10540.1.S1_at	79	TATTGATACAGAGGGCAGTTTC	AGCTGCATATTCGGAAGTAG	173
RAD54	Ta.7706.1.S1_at	82	GCTGTCGCAAACCCTTTCG	GTCGTAGGCACCAATCATCCATC	344
RPA	Ta.6986.1.S1_at	83	GAATGTCTTCCGTGAACCG	CATCTAAGGACGGGTGCTAG	274
WM5	Ta.25342.1.S1_at	81	CATCGAGTGGGCTTTAGCTATAG	CAACACGTAGTACGATAGATCCAAG	178
WMC3*	Ta.28776.1.A1_at	79	CGACCGACGGAATTGATATG	CTGTGAGAAGTTTCAGCATTATC	171
WMC4	Ta.30383.1.A1_at	80	GATTCCCAAATGTTCGAC	CAAATTGTCCTCCTTACG	266
WMC5	Ta.3224.2.S1_at	81	CTTGCTGCTCCCAATGATAATTC	CAGTTAGCTGCCAGGTCATCTTTC	245
WMC6	Ta.3385.1.A1_at	80	CGGACAGTCCAGATGTGC	CTATGCTTGCTGCCTGATG	271
WMC10*	TaAffx.38162.1.S1_at	79	AGGAGTTGACATGACAAGATTAGGGAGG	GCCACTTCACATGCACCCATCTAATC	166
Controls
Cyclophilin		82	CAAGCCGCTGCACTACAAGG	AGGGGACGGTGCAGATGAA	227
EFA1		82	CAGATTGGCAACGGCTACG	CGGACAGCAAAACGACCAAG	227
GAPdH		78	TTCAACATCATTCCAAGCAGCA	CGTAACCCAAAATGCCCTTG	220
Actin		77	GACAATGGAACCGGAATGGTC	GTGTGATGCCAGATTTTCTCCAT	236
Flat gene 1	Ta.9657.1.S1_at	83	CCCTCAGACCGCTGGCAA	GAGGCGGACAGACATGGAAGAA	300
Flat gene 2	Ta.28350.1.S1_a_at	80	ACGCAGCTACCTGTATCATTCGGATC	GAAGCGACGATGTCCACATGACC	160

Primer sets used and the product sizes obtained in addition to the acquisition temperatures used. Control genes in italics were those selected by geNorm for normalisation.
* indicates those transcripts that have an unclear orientation (not known whether sense or antisense).

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ISSN: 1471-2164

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