Figure 5

Time-lapse recordings of the expression level of hh -related genes. (A) One transgenic larva carrying Pgrl-4::GFP was recorded starting from late L2 to late L3. There is no apparent change in the expression level before and after molt. (B, B') One transgenic larva carrying Pwrt-9::GFP was recorded starting from middle L1 to late L2. The GFP signal is high 3–4 hr before the L1 molt and then goes down and reaches a valley 3–4 hr after the L1 molt and then increases again. A graph representing the intensity change was generated from the raw image data as described in Methods. (C) One transgenic larva carrying Pwrt-10::GFP is recorded starting from mid-L2 to mid-L3. The GFP signal is high 5–6 hr before the L2 molt. It decreases and reaches its lowest level around 2 hr after the L2 molt and then increases again. In all the three panels, the time interval between images is 1 hr.