Table 1 Bacterial strains and plasmids

Escherichia coli

DH5α

F^-, φ80dlacZ ΔM15 Δ(lacZYA-argF)U169

Invitrogen

deoR recA 1 endA 1 hsdR 17(rk^-, mk⁺)

phoA supE 44 λ^- thi-1 gyrA 96 relA 1

S17-1

recA pro hsdR RP4-2-Tc::Mu-Km::Tn7

[45]

Pseudomonas aeruginosa

PAO1

Wild type

[46]

PAO1ΔlasR

PAO1 derivative; ΔlasR::Tc^R

[47]

PAO1ΔrhlR

PAO1 derivative; ΔrhlR::Gm^R

[47]

PAO1ΔrhlR ΔlasR

PAO1 derivative;

ΔrhlR::Gm^R

ΔlasR::Tc^R

[47]

PAO-rpoS

PAO1 derivative; rpoS::Gm^R

[15]

MSC100

PAO1 derivative; ΔrhlR ΔlasR, pBADlasR

This study

MSC110

PAO1 derivative; ΔrhlR ΔlasR, pBADrhlR

This study

MSC120

PAO1 derivative; rpoS::Gm^R, pBADrpoS

This study

Plasmids

pFLP2

Source of Flp recombinase; Ap^R

[37]

pJN105

araC-pBAD cloned in pBBR1MCS-5; Gm^R

[41]

pJN105.lasR

lasR in pJN105

[48]

pJN105.rhlR

rhlR in pJN105

This study

pJN105.rpoS

rpoS in pJN105

This study

pSW196

mini-CTX1 based plasmid containing

[40]

araC-pBAD from pBAD30; Tc^R

pSW196.lasR

lasR in pSW196

This study

pSW196.rhlR

rhlR in pSW196

This study

pSW196.rpoS

rpoS in pSW196

This study