MELOGEN: an EST database for melon functional genomics

BMC Genomics

Table 8 Potential melon miRNAs

Name	Potential mature miRNA sequence (5'->3')*	Melogen unigene^†	Precursor folding free energy (kcal mol^-1)^§	MFEI (kcal mol^-1)^Δ	miRNA family	Potential target in melon
m2	ugaagcugccagcaugaucu	bCL2353Contig1	-206.8	-0.67	miR167	--
m4	ugauugagccgugccaauauc	bPSI_40-F10-M13R_c	-195.0	-0.66	miR171	bHS_39-C12-M13R_c
m7	ucggaccaggcuucauucccc	bA_31-D02-M13R_c	-160.8	-0.70	miR166	--
m8	uugacagaagauagagagcac	bCI_04-H02-M13R_c	-188.2	-0.74	miR157	bCI_30-A09-M13R_c
m12	uuggacugaagggagcucccu	b15d_24-H05-M13R_c	-163.6	-0.86	miR319	bCL2243Contig1

*Nucleotide sequences correspond to potential mature miRNA sequences as deduced from BLAST searches using known plant miRNA sequences [54] and analysis of secondary structure predictions [52]. ^†Accession numbers (MELOGEN database) of potential precursors of melon miRNAs are given. ^§Computational values of folding free energies have been calculated using MFOLD 3.1 [55]. ^ΔMinimal folding free energy indexes (MFEIs) have been calculated as described by Zhang et al. [53].

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