Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus

Mayhew, George F; Bartholomay, Lyric C; Kou, Hang-Yen; Rocheleau, Thomas A; Fuchs, Jeremy F; Aliota, Matthew T; Tsao, I-Yu; Huang, Chiung-Yen; Liu, Tze-Tze; Hsiao, Kwang-Jen; Tsai, Shih-Feng; Yang, Ueng-Cheng; Perna, Nicole T; Cho, Wen-Long; Christensen, Bruce M; Chen, Cheng-Chen

doi:10.1186/1471-2164-8-462

Table 2 Clusters showing significant differences as determined by Stekel R value and Chi square analysis.

From: Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus

GBID	Product	asuhem	diroinf	imacbac	brumal	naïve7	naïve14	R	Chi
EU204979	NADH dehydrogenase subunit 2	245	23	14	10	21	19	0	0
EU204980	NADH dehydrogenase subunit 6	172	18	8	1	10	11	0	0
EU204999	cytochrome c oxidase subunit I	245	67	24	11	59	54	0	0
EU205005	unknown	325	52	33	17	26	66	0	0
EU205016	cytosolic small ribosomal subunit S26	60	69	57	41	36	67	0.000001	0.000001
EU205017	NADH dehydrogenase	28	2	2	4	6	1	0.000002	0.000001
EU205057	ubiquitin	83	0	0	0	1	0	0	0
EU205058	cytochrome c oxidase subunit III	277	79	83	20	62	52	0	0
EU205059	cytochrome b	47	19	14	1	9	8	0	0
EU205061	mitochondrial adenosine triphosphatase subunit 6	202	60	36	1	51	45	0	0
EU205096	ubiquinol-cytochrome c reductase subunit	62	9	4	12	9	11	0	0
EU205097	cytochrome c	74	15	8	12	11	12	0	0
EU205100	unknown	243	59	123	79	54	72	0	0
EU205101	putative: conserved unknown	32	18	7	3	20	6	0.000001	0.000002
EU205152	ubiquinol-cytochrome c reductase	56	14	5	11	14	9	0	0
EU205153	AT DNA binding	0	7	15	13	6	8	0.000002	0.00033
EU205154	questionable: Bombyx mori prophenoloxidase activating factor 3	26	1	3	3	1	1	0.000001	0
EU205155	questionable: conserved unknown	1	6	7	21	8	10	0.000006	0.00008
EU205171	ubiquinol-cytochrome-c reductase	94	16	8	29	20	26	0	0
EU205182	cytosolic large ribosomal subunit L36	27	43	33	24	26	47	0	0
EU205185	NADH dehydrogenase	47	2	8	10	4	7	0	0
EU205197	gelsolin	129	20	38	33	13	57	0	0
EU205226	cytosolic large ribosomal subunit L40	21	5	0	0	5	1	0	0.000005
EU205248	cytochrome C oxidase subunit II	52	15	12	6	9	27	0.000001	0.000002
EU205296	Myosin alkali light chain 1	35	3	8	0	3	6	0	0
EU205304	questionable: receptor binding	0	0	0	2	6	0	0.000228	0.000002
EU205306	questionable: cytochrome c oxidase	63	13	6	6	13	3	0	0
EU205324	conserved unknown	17	0	4	0	0	4	0.000003	0.000054
EU205330	cytosolic small ribosomal subunit S9	39	73	43	68	32	54	0	0
EU205338	superoxide dismutase	20	2	1	0	1	1	0.000002	0.000001
EU205349	ribosomal protein L41	276	100	170	94	126	104	0	0
EU205374	nucleoside diphosphate kinase	2	14	1	11	0	5	0.000002	0.000002
EU205385	questionable: protein-tyrosine kinase	0	0	0	107	0	0	0	0
EU205393	hydrogen transporting two sector ATPase	53	11	11	8	17	9	0.000003	0.000002
EU205409	cecropin	17	1	1	0	0	5	0.000006	0.000023
EU205450	serine protease	1	6	28	9	3	19	0	0
EU205451	cytosolic small ribosomal subunit S3A	78	26	27	16	20	29	0.000068	0.000132
EU205465	defensin	136	14	27	6	5	9	0	0
EU205470	putative: heat shock	0	0	1	56	2	3	0	0
EU205505	lysozyme	116	17	32	10	11	35	0	0
EU205526	cytochrome c oxidase	130	15	11	10	16	5	0	0
EU205556	putative: conserved unknown	0	0	3	41	0	3	0	0
EU205557	putative: conserved unknown	0	0	0	9	0	1	0.000047	0.000003
EU205560	cecropin	32	35	17	3	14	11	0	0
EU205570	putative: conserved unknown	0	9	13	15	7	7	0.000003	0.000718
EU205592	conserved unknown	63	21	11	12	12	16	0.000007	0.000004
EU205632	putative: calcium ion binding protein	0	0	13	0	0	0	0	0
EU205658	serine protease	112	18	42	43	7	29	0	0
EU205659	unknown	35	0	3	0	2	0	0	0
EU205708	proton-transporting ATP synthase complex subunit	70	8	9	4	14	16	0	0
EU205709	putative: conserved unknown	25	0	3	2	2	2	0.000001	0
EU205750	hydrogen-exporting ATPase	33	2	5	2	1	9	0	0
EU205751	putative: odorant-binding	0	0	0	9	0	0	0.000015	0
EU205764	questionable: odorant-binding protein 56e	0	0	0	9	0	0	0.000015	0
EU205767	trypsin	0	0	0	25	0	0	0	0
EU205782	trypsin	10	221	98	70	107	70	0	0
EU205800	unknown	5	4	9	7	11	39	0	0
EU205806	questionable: odorant-binding	0	0	0	123	0	0	0	0
EU205807	questionable: odorant-binding	0	0	0	28	0	0	0	0
EU205828	trypsin	3	85	10	11	33	2	0	0
EU205832	questionable: apolipophorin	129	0	5	32	2	6	0	0
EU205858	vitellogenin C	0	0	0	18	0	0	0	0
EU205893	unknown	130	0	0	0	0	1	0	0
EU205915	hydrogen transporting two sector ATPase	41	6	5	2	2	4	0	0
EU205924	putative: serine protease	5	13	28	2	14	6	0	0
EU205925	putative: serine protease	1	15	10	4	12	5	0.000001	0.000002
EU205991	unknown	0	3	1	39	2	4	0	0
EU205996	ATPase synthase	16	4	1	0	0	0	0.000003	0.000012
EU206001	NADH dehydrogenase	36	6	3	8	7	1	0.000001	0.000001
EU206048	conserved unknown	45	8	11	10	4	6	0.000021	0.000005
EU206083	putative: serine protease	0	0	0	61	0	0	0	0
EU206123	putative: serine protease	0	5	11	28	7	8	0	0
EU206138	questionable: conserved unknown	0	0	0	32	0	0	0	0
EU206244	cytosolic large ribosomal subunit L34a	1	38	10	9	7	3	0	0
EU206248	zinc-metalloproteinase precursor	2	14	1	0	12	3	0	0
EU206376	putative: cathepsin	0	0	0	30	0	0	0	0
EU206422	infection responsive short peptide (gambicin)	17	23	40	47	17	24	0.000001	0.000002
EU206504	unknown	0	0	0	12	0	0	0	0
EU206507	ATP/ADP antiporter (transporter)	21	3	1	0	1	0	0	0
EU206546	beta-globin	0	0	0	34	0	0	0	0
EU206601	cathepsin	0	0	0	19	0	0	0	0
EU206635	trypsin	0	4	0	16	3	2	0	0
EU206733	putative: conserved unknown	0	0	0	9	0	0	0.000015	0
EU206824	putative: arrestin 2	0	13	6	8	9	9	0.000004	0.000307
EU206866	questionable: conserved unknown	0	0	0	32	0	0	0	0
EU206900	rhodopsin	0	2	13	11	5	11	0.000001	0.000066
EU206907	questionable: chitin binding	0	0	0	12	0	0	0	0
EU206986	unprocessed 18S ribosomal RNA	40	1	0	1	0	0	0	0
EU207626	unknown	90	0	0	1	0	2	0	0
EU207634	unknown	107	56	65	38	22	89	0	0
EU207658	NADH dehydrogenase subunit	26	2	2	1	8	5	0.000003	0.000006
EU207722	questionable: triacylglycerol lipase	0	0	0	8	0	0	0.000066	0.000002
EU207765	putative: odorant-binding protein G.1A.F	0	0	0	22	0	0	0	0
EU207810	putative: trypsin	0	0	0	21	1	0	0	0
EU207816	hydrogen transporting two sector ATPase	48	8	8	6	8	13	0.000004	0.000001
EU207921	vitellogenin	0	0	0	19	0	0	0	0
EU207965	defensin	17	2	5	0	0	0	0.000002	0.00002
EU208023	questionable: threonine-rich salivary mucin	0	0	0	0	6	4	0.000031	0.000003
EU212979	NADH dehydrogenase	36	5	10	0	4	3	0	0

Clusters and their constituent ESTs were analysed using IDEG6 [20] to find clusters where the number of ESTs were statistically different between the libraries. Of 8,020 clusters analysed, 99 showed a significantly differential number of ESTs collected from one or more of the libraries. Each row represents the Genbank Accession number for cluster, and the columns are the number of ESTs from each of the six libraries that are a member of it. The libraries are: asuhem (bacteria-inoculated, hemocyte), diroinf (whole body, Dirofilaria immitis injected), imacbac (bacteria-inoculated whole body), brumal (blood-fed Brugia malayi), n7 and n14 (naïve, 5–7 and 12–14 days of age). The R value is the inverse log of the Stekel R Score [23], and the Chi value is a general Chi square analysis.

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ISSN: 1471-2164

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