Figure 5

Analysis of Gene Ontology (GO) terms. Genes were categorized as significantly differentially expressed in a two-way comparison with false discovery rate correction and p < 0.05, and information about associated GO terms was extracted using Blast2GO [39]. Fisher's exact test was used to calculate the probability that the observed distribution of terms in the test set was significantly different (p-value < 0.05) from the distribution of all assembled sequences for the following comparisons: A) females vs. males; B) males vs. females; C) juvenile females vs. mature females; D) mature females vs. juvenile females; E) mature females vs. males; F) Daphnia and Drosophila conserved sex-biased genes. GO terms for each column are: 1, chromatin; 2, nucleosome; 3, nucleotide binding; 4, microtubule cytoskeleton organization and biogenesis; 5, lytic vacuole; 6, pattern binding; 7, nucleic acid binding; 8, DNA binding; 9, structural constituent of ribosome; 10, translation elongation factor activity; 11, catalytic activity; 12, GTPase activity; 13, endopeptidase activity; 14, serine-type endopeptidase activity; 15, chymotrypsin activity; 16, trypsin activity; 17, hydrolase activity, hydrolyzing O-glycosyl compounds; 18, chitinase activity; 19, structural molecule activity; 20, GTP binding; 21, cellular component; 22, extracellular region; 23, intracellular; 24, cell; 25, nucleus; 26, chromosome; 27, cytoplasm; 28, lysosome; 29, vacuole; 30, endoplasmic reticulum; 31, ribosome; 32, microtubule; 33, carbohydrate metabolism; 34, polysaccharide metabolism; 35, chitin metabolism; 36, nucleobase, nucleoside, nucleotide and nucleic acid metabolism; 37, DNA packaging; 38, establishment and/or maintenance of chromatin architecture; 39, chromatin assembly or disassembly; 40, nucleosome assembly; 41, transcription; 42, transcription, DNA-dependent; 43, regulation of transcription, DNA-dependent; 44, protein biosynthesis; 45, translational elongation; 46, protein complex assembly; 47, proteolysis and peptidolysis; 48, amino acid and derivative metabolism; 49, amino acid metabolism; 50, aromatic compound metabolism; 51, organelle organization and biogenesis; 52, chromosome organization and biogenesis (sensu Eukaryota); 53, cytoskeleton organization and biogenesis; 54, microtubule-based process; 55, physiological process; 56, chitin binding; 57, translation factor activity, nucleic acid binding; 58, biological_process; 59, metabolism; 60, peptidase activity; 61, serine-type peptidase activity; 62, catabolism; 63, macromolecule catabolism; 64, biosynthesis; 65, macromolecule biosynthesis; 66, cellular process; 67, microtubule cytoskeleton; 68, small ribosomal subunit; 69, cell organization and biogenesis; 70, hydrolase activity; 71, hydrolase activity, acting on glycosyl bonds; 72, isomerase activity; 73, purine nucleotide binding; 74, guanyl nucleotide binding; 75, regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism; 76, regulation of metabolism; 77, protein metabolism; 78, protein catabolism; 79, carbohydrate binding; 80, polysaccharide binding; 81, ribonucleoprotein complex; 82, microtubule polymerization or depolymerization; 83, structural constituent of cuticle; 84, translation; 85, macromolecule metabolism; 86, organelle; 87, membrane-bound organelle; 88, non-membrane-bound organelle; 89, intracellular organelle; 90, intracellular membrane-bound organelle; 91, intracellular non-membrane-bound organelle; 92, protein complex; 93, biopolymer metabolism; 94, biopolymer catabolism; 95, cellular metabolism; 96, primary metabolism; 97, cellular catabolism; 98, cellular biosynthesis; 99, cellular protein catabolism; 100, cellular macromolecule metabolism; 101, cellular carbohydrate metabolism; 102, cellular polysaccharide metabolism; 103, cellular macromolecule catabolism; 104, cellular protein metabolism; 105, translation regulator activity; 106, tubulin; 107, regulation of transcription; 108, microtubule polymerization; 109, regulation of biological process; 110, regulation of physiological process; 111, cellular physiological process; 112, protein polymerization; 113, chromosome organization and biogenesis.