Table 4 Oligonucleotide primers used for microarray results validation with qRT-PCR
From: Transcriptional profiling of Actinobacillus pleuropneumoniae under iron-restricted conditions
# | Gene | Forward Primer | Reverse Primer |
|---|---|---|---|
| Â | ackA | CCTAAAACGGGTGACGAGAA | ACCGATAGCACCGATACTGG |
1 | ap1465 | CGTAGCGCGTTCCGAATTAA | AACTGCCGTATTTGTCGTGC |
2 | apxIC | TGGTTATGGGCAAGTTCTCC | CAACTAGCGAGGCAACATCA |
3 | ccmC | ATACGGTTCTATGGCGGTTG | AAACAACACCAAAGCCGAAG |
4 | dcuB2 | GGCTTTGAAGGCGTTACACT | GCCGGTAATTGCTCGTCTAA |
5 | dmsA | AACTGTGGTAGCCGTTGTCC | AATGCGGCAAACTGATAACG |
| Â | exbB1 | CCGTTCATTGGGTTATTTGG | ACGGTTAAGGCGAGCAATTA |
| Â | exbD1 | GGGCATTTATTTAGGCGAGA | TGAGTCACAAAGCCTATTTTCG |
6 | fetB2 | CCGCTCTTGATATTCCGATG | TTCCAAGCGTTTGTTTGATG |
7 | hgbA | TGAATTTCGGGCAATTATGG | TCCGCTTTCTTCGCACTTAC |
8 | NMB1668 | AAACGGATTTCGGCATACAC | CGTACCGGAGAACATTTCGT |
9 | nrfA | AAGAAAAACCGGCTCAAACA | ATAACCCGCCCATAACACAA |
10 | nrfC | GCACCCGTAGAGACTTCGTC | GCCTTCCGGTACTTTGTTTG |
11 | nrfE | CCGTTTGAGCGTAGTTTTCC | ATTGTCCAAGGTCGAATCCA |
12 | omp64 | GCGGACAGTAAGCCTGAAAC | TGTTGTCGCATTTGAACCAC |
13 | ompW | GGCGAAGTGGCAAAAGTAAA | CAACACCTAAATTCGCATCG |
14 | pepD | GGCGCAAAAGTAGCATTCTC | TTGTCGGTCCGATAGAAACC |
15 | PM0741 | GGCTCGGATTCATTTACCAC | AATAGACCGCATCCAGCTTC |
| Â | tbpA | ATTGGCAACCATCGGATTTA | GCACCTAAGCGATCACGAGT |
16 | tonB1 | CTCCCTTGGTGCTGGTTATG | AATTTTTGCCGGTTGATACG |
17 | torA | GAATTTCCTTGTGCCGAGAG | GCTTCGCCGTATACCAAGTC |