Figure 1

Diagram illustrating the in vitro infection strategy, tetracycline clearing and microarray analysis of infected (S2_I) and uninfected (S2_U) cell lines. The line illustrates an estimated infection level based upon periodic qPCR assays (indicated by hollow circles). The number provides a relative measure, not the absolute number of Wolbachia. The "waves" in the left half of the graph resulted from removal of the infection by host and enrichment of infection by shell vial introduction. Arrows indicate shell vial introduction of Wolbachia infection. At passage 17 (marked with asterisk), the cell line was split and one subline was tetracycline treated. At passage 23 (grey shading) the cell line was examined via microarray.