Developing and applying a gene functional association network for anti-angiogenic kinase inhibitor activity assessment in an angiogenesis co-culture model

Table 1 Gene Ontology (GO) terms enriched with differentially expressed genes in HUVEC of different passages

GO¹	GO Term	DEG²	FDR³
Biological Process	cell cycle	93	0.00075
	mitotic cell cycle	44	0.00042
	M phase of mitotic cell cycle	36	0.00124
	programmed cell death	44	0.00717
	mitosis	35	0.00511
	cell division	37	0.00332
	regulation of cell cycle	60	0.01370
	cell cycle checkpoint	13	0.01512
	cell organization and biogenesis	113	0.05000
	cell proliferation	51	0.01164
Molecular Function	protein binding	270	0.00051
	ATP binding	106	0.00048
	nucleotide binding	137	0.00473
	catalytic activity	309	0.00144
	purine nucleotide binding	119	0.00210
	cytoskeletal protein binding	33	0.00545
	kinase activity	70	0.03651
	binding	545	0.03367
	transferase activity	116	0.01551
Cellular Component	spindle	14	0.00058
	microtubule cytoskeleton	36	0.00182
	intracellular	439	0.00138
	cytoskeleton	73	0.00558
	nucleus	237	0.00615
	intracellular membrane-bound organelle	317	0.04183
	intracellular organelle	364	0.01793

1. Gene Ontology; 2. Differentially expressed genes; 3. False discovery rate
Listed are GO categories identified through enrichment testing of the differentially expressed genes from HUVEC using the DAVID functional annotation system as described in the methods section.

ISSN: 1471-2164