Figure 4From: Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNAIdentification of the protein binding regions in DNA fragments by a combination of EMSA and denaturing PAGE. (A) L and H molecular fractions of corresponding Bsu RI and Alu I fragments were incubated with (lane +) and without (lane -) PC12 nuclear extract (NE) and analysed by EMSA. Direct phosphoimaging of the wet 1 mm-thick gel is shown. (B) Denaturing PAGE of the shifted fragments. Lanes - and + show the pool of fragments used in EMSA and shifted bands (smear), respectively. Connecting lines indicate the bands or smear analyzed on two different gels. M, 32P-labeled 100 bp ladder (Gibco-BRL).Back to article page