Figure 3

Relative steady state mRNA levels of selected trypanosome genes. Fluorescence intensity data for eight microarray experiments comparing BSF to PCF, representing four biological replicates plus dye-swaps, was normalised to the total signal intensity of each slide, averaged and plotted as bar-graphs against ORF designation or accession number. Error bars indicate the standard deviation, dark bars are BSF and light bars PCF. Panel A: Small GTPases. ARL and Rab annotations are based on [39], Ras-like annotations based on [29] and additional data at GeneDB. Panel B: Cell surface proteins. Panel C: SNARE family proteins. Annotations based on domain architecture and similarity to sequences described for L. major [59]. Panel D: Putative secretory pathway proteases. ORFs were included for predicted proteases that bear an ER targeting sequence and/or a predicted trans-membrane domain. In each cohort, a limited number of transcripts are highly expressed.