Figure 5

Alignment of the myo-2 enhancer and corresponding web page view. Left: Functional subelements of the myo-2 enhancer are highlighted by yellow boxes. The cell-type-specific subelement B207, which is identical in all species, binds and is activated by the pharyngeal muscle specific NK-2 family homeodomain factor CEH-22 [24] [29]. The organ-specific subelements C181 and C183 bind and are activated by the pan-pharyngeal FoxA family transcription factor PHA-4 [30], which is required for formation of pharyngeal muscle and all other pharyngeal cell types during embryonic development. The C elements are a little less conserved than B207, but the PHA-4 binding site matches the high-affinity consensus sequence TGTTTRC [31]. Right: Web page view of the same genomic region. The high-affinity consensus sequence TGTTTRC for PHA-4 binding is highlighted in red.