Table 3 Allelic variation in 39 SSR loci.
From: Gene-based microsatellite development for mapping and phylogeny studies in eggplant
Code | Repeat | FORWARD PRIMER (5'-3') | REVERSE PRIMER(5'-3') | Expected size of alleles (bp) | Allele size range (bp) | Nr. of alleles1 | PIC2 | SSR position3 | ||
|---|---|---|---|---|---|---|---|---|---|---|
NA1 | NA2 | PICm | PICs | |||||||
EEMS06 | (T)14 | TCATGCGAAGATTAATTAAATGTGA | GAGTGGATGATCAAGAATGGC | 265 | 268–274 | 1 | 4 | 0 | 0,174 | 3' UTR |
EEMS07 | (T)13 | CCATGCCAGAATGGAAACTT | AACGAAAACACGATCAACCC | 247 | 250–260 | 1 | 4 | 0 | 0,209 | 3' UTR |
EEMS10 | (A)20 | TCAAGCAGAACGAAGATGGA | GTAGGGGACGTGGATTCAGA | 282 | 266–290 | 1 | 4 | 0 | 0,174 | 3' UTR |
EEMS12 | (A)16 | CGGGCAACTCTTCACATTTT | ATTGGTTTGCTATCGAATTTCT | 158 | 146–150 | 2 | 3 | 0,097 | 0,273 | 5' UTR |
EEMS13 | (A)14 | TGAGATACGCGTACAATGACTTC | GGGGTTTTGCTGCTGTTATC | 140 | 140 | 1 | 1 | 0 | 0 | 5' UTR |
EEMS14 | (A)13 | GGAATGGACCAAACCCCTAA | AGAGCTTCGTTGCTTGGTGT | 277 | 270–276 | 1 | 3 | 0 | 0,088 | 5' UTR |
EEMS15 | (C)12 | GGGACAAATCTGACCTTTGG | CTGGTGGCAAATTCTTCGAT | 292 | 270–294 | 5 | 7 | 0,645 | 0,755 | 3' UTR |
EEMS16 | (AC)7 | CAATTTTTCGGTTCACTAATCAAG | CTTCAAGGAAAAAGGAGGCC | 132 | 135–141 | 3 | 4 | 0,135 | 0,260 | 3' UTR |
EEMS17 | (CA)8 | TGACATGTAGCTGGGCAGAG | TGGAGTGTGCATCCCAAATA | 197 | 195–197 | 2 | 2 | 0,492 | 0,499 | 3' UTR |
EEMS18 | (AG)7 | GGAGAAACTGAAAAATTTGTAGAGAG | GAGGAGTTTCCGACATGAGC | 187 | 183–187 | 1 | 3 | 0 | 0,126 | CDS |
EEMS19 | (AT)9 | GGCATGACAAAATCATACAAACA | TGTTGGTTAAGTCCATGGGAA | 173 | 165–177 | 1 | 3 | 0 | 0,135 | 3' UTR |
EEMS20 | (AT)8 | AACATCAGCCAGGGTGTTTC | TGCTGAAAATTACAAGCCAAA | 215 | 221–227 | 2 | 4 | 0,049 | 0,278 | 3' UTR |
EEMS21 | (AGA)5 | TGATGTTGAACCGACACAAGA | CGTCTTCATCTTCCTCCTCG | 131 | 122–140 | 1 | 3 | 0 | 0,126 | CDS |
EEMS22 | (AAG)5 | GAAGGACGTTGGTCCTGGTA | CTGTTCATTATCCCCATCGC | 162 | 165–168 | 1 | 2 | 0 | 0,085 | CDS |
EEMS23 | (TTC)5 | CACCAATTTCCCCCTTCTTT | CGGTTGGTAAAGAAAACCCA | 144 | 145 | 1 | 1 | 0 | 0 | CDS |
EEMS24 | (CTT)5 | CACCTGTTTGAGCACCTTGA | CACCGAAGGCAGAGAAGAAG | 221 | 217–220 | 1 | 2 | 0 | 0,043 | CDS |
EEMS25 | (CTT)5 | CCCATAGCTTTGCTCGAGAT | GCACCAAAGGCAGAGAAGAA | 227 | 225–230 | 1 | 2 | 0 | 0,043 | CDS |
EEMS26 | (CTT)5 | GACACTCCCCTACTTCCACCT | CGCTTAGCAGAAGCCGATAA | 260 | 355–360 | 1 | 2 | 0 | 0,087 | CDS |
EEMS28 | (TAA)21 | GACGATGACGACGACGATAA | TGGACTCACAACTCAGCCAG | 219 | 180–236 | 7 | 9 | 0,665 | 0,714 | 3' UTR |
EEMS29 | (ATG)5 | TCAGTCAACTGCATCACCAGA | ATTCCCATTATTGGCTGCTG | 118 | 120 | 1 | 1 | 0 | 0 | CDS |
EEMS30 | (TAC)5 | TTTACATGACAGCACCAGGC | ATTTTATGGGAATGGGGTCC | 191 | 189–195 | 1 | 2 | 0 | 0,087 | 3' UTR |
EEMS31 | (TGG)5 | GAGAAGTTGGCTTCAGTGCC | TAAACTCAAGGGATGCTGGG | 239 | 330–339 | 1 | 2 | 0 | 0,043 | CDS |
EEMS32 | (TCA)5 | TAAGGAGTCTGATGCCGCTT | GTAATGCTCCTCCACGGCTA | 151 | 150 | 1 | 1 | 0 | 0 | CDS |
EEMS33 | (TCA)5 | CTATCTCCTTTTCCCCGACC | ATGAATAAGCTGCCACCACC | 220 | 222 | 1 | 1 | 0 | 0 | CDS |
EEMS34 | (TCA)5 | GCTTGATTCCCCACAAAGAA | GTTTCATCGCCCTCATCATT | 276 | 275–278 | 2 | 2 | 0,123 | 0,143 | CDS |
EEMS35 | (TCA)5 | ATGGCTTCTGATGGACCAAG | CACTTGATGAACGTGGATGG | 230 | 232 | 1 | 1 | 0 | 0 | CDS |
EEMS36 | (TGT)5 | TCTATCATCCCCAGATCCCA | AAGGTCGCATGGACATTAGG | 117 | 110–120 | 1 | 2 | 0 | 0,043 | CDS |
EEMS37 | (TCC)5 | CCCTTCCTACCCACACTTCA | GTTTTGCACCTTTCCATCGT | 117 | 114–123 | 2 | 4 | 0,375 | 0,502 | CDS |
EEMS38 | (CAC)5 | TTCAATCGAACTTCGGAACC | ATGACGGTGGATCTCGCTAC | 148 | 135–153 | 1 | 3 | 0 | 0,086 | CDS |
EEMS39 | (CTG)5 | GGAGAGATGGATGCCGAATA | TCTCGACCTTAGCCTGCATT | 166 | 264–270 | 1 | 3 | 0 | 0,126 | CDS |
EEMS41 | (GCA)5 | ATTCTGCATTCATCGGAAGG | GGATTGCTTGTGGGAATATCA | 260 | 700, 1600 | 1 | 1 | 0 | 0 | CDS |
EEMS42 | (GCA)6 | GCTCAGCAACCACAGTACCA | GTCCGGACTTCATCAGCATT | 152 | 155–180 | 1 | 3 | 0 | 0,166 | CDS |
EEMS44 | (GCC)5 | CCTTCAAACCCTCTCCCTTC | GTGAAACGTGGTGGAGGTCT | 216 | 215 | 1 | 1 | 0 | 0 | CDS |
EEMS45 | (AGAACC)4 | AGCGCTTGTCCAGGCTATAA | TTTCCACCATGAGCAAATGA | 282 | 279–285 | 1 | 2 | 0 | 0,197 | |
EEMS46 | (ACCAGC)6 | ACCAAACGTGCATGAAACAA | GGAAATGTTGGTGGAATTGG | 264 | 245–265 | 1 | 4 | 0 | 0,207 | CDS |
EEMS47 | (GCT)5..(TTC)5 | CGAACACATTCGCAAATCAC | GCATCACAAGGATGGAAAGG | 246 | 250–253 | 1 | 2 | 0 | 0,162 | CDS |
EEMS48 | (TAA)20(CGA)8 | CAATGCAAACAATTATCATTTCG | TCGATGTTGTTGTCGTCGTT | 213 | 223–241 | 7 | 9 | 0,641 | 0,677 | 3' UTR |
EEMS49 | (TA)12(GA)7 | TGAAATTGATCAATACCTATAAATTTAG | GAAAGCCAGGATAGCATTCG | 140 | 145–153 | 5 | 5 | 0,677 | 0,677 | 3' UTR |
EEMS50 | (TA)9(GA)8 | AAATCCGGCCATTCTGTGTA | ACATCGTTCCGCCTCTATTG | 224 | 218–226 | 2 | 4 | 0,229 | 0,377 | 5' UTR |