Figure 5From: Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciaeCulture supernatant proteins from extracellular protein secretion system mutants. Cells were grown in Y-mannitol medium for 4 days. Proteins were precipitated with trichloroacetic acid. A, R. l. bv. viciae 3841 wild type; B, toaD mutant; C, tobD mutant; D, prsD mutant; E, virB6 mutant; F, autA mutant; G, autB mutant; H, autC mutant. Protein bands missing in the lane representing the prsD mutant are marked (*).Back to article page