Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciae

BMC Genomics

Table 2 Culture supernatant proteins from R. l. bv. viciae 3841 were identified by MALDI-ToF MS.

Band	Apparent weight [kDa]	Top MOWSE score	Number of matched fragments	Predicted protein	Theoretical weight [kDa]	Predicted function
*1	89.1	205	18	RL2961	95.0.	cadherin-like protein
*2	59.2	165	15	pRL90140	79.0.	parallel beta-helix repeat protein
*3	57.7	113	15	pRL100309	60.7	cadherin-like protein
*4	52.2	78	8	RL2412	64.6	glycosyl hydrolase
5	50.2	243	20	RL4651	63.7	dipeptide-binding protein
6a	46.5	55	7	RL0778	58.8	dipeptide-binding protein
*6b	46.5	195	16	RL3024	51.6	PlyB
7	41.5	101	10	RL0728	44.4	flagellar hook
8	38.5	174	14	RL4218	47.6	sorbitol-binding protein
9	37.5	76	8	RL3329	43.4	membrane bound lytic tranglycosylase
10	35.6	77	7	RL3745	38.9	Leu/Ile/Val-binding protein (BraC)
11a	34.1	60	6	RL0720	33.6	flagellin
11b	34.1	96	9	RL2375	38.9	basic membrane lipoprotein
*12a	29.8	58	5	pRL100451	24.9	RapA2
12b	29.8	60	6	RL0196	35.0.	basic membrane lipoprotein
12c	29.8	44 (53)	5	RL0489	35.4	sugar-binding protein
12d	29.8	36 (51)	4	RL0718	31.0.	flagellin
13	27.7	129	10	RL0518	39.4	ribose-binding protein
14	25.0.	113	9	RL2753	27.9	arginine/ornithine-binding protein
15	20.4	57	5	RL1369	24.5	pentapeptide repeat protein
16	17.1	85	6	RL2404	20.5	peptidyl prolyl cis-trans isomerase
*17	16.3	83	6	RL1580	15.3	nucleoside diphosphate kinase
18	16.0	70	5	RL2697	19.5	COG3184

The protein bands labelled in Fig. 6. were cut out from the gel and digested with trypsin. The masses of the tryptic digest were identified by MALDI-ToF MS and searched against a R. l. bv. viciae 3841 genome database. Band #, protein band as labelled in Fig. 6., Band from which more than one protein was identified are indicated (e.g. 6a, 6b). Apparent weight, molecular weight of the proteins in the given band as estimated from the electrophoretic mobility. Top MOWSE score, statistical score calculated by the MASCOT program for the R. l. bv. viciae 3841 indicated in column 'predicted protein', scores above 50 indicate >95% confidence. Number of matched fragments, number of fragment masses which could be assigned to the predicted protein. Predicted protein, proteins scoring more than 50 as MOWSE score. Theoretical weight, calculated molecular weight of the indicated proteins. Scores obtained after refining search parameters are shown in brackets. An asterisk indicates PrsDE-dependence.

ISSN: 1471-2164