Table 2 Validation of differential gene expression observed in microarray experiments via qPCR.
Gene | mRNA level change | Sense primer sequence (5'-3') | Antisense primer sequence (5'-3') | |
|---|---|---|---|---|
| Â | qRT-PCR | microarray | Â | Â |
SAN_1753 | -2.1 | -2.4 | GGGTTGGGAAGGTGCTTAC | TAAACCTCCAATAAGGAACGAC |
SAN_1754 | -3.0 | -2.4 | ATGGAATTGTCGGTTATTCAGGAG | GTCAGTGTCACCTTGTTGTCG |
SAN_1756 | -6.5 | -5.3 | GCTCCAATTCGTATCTATTCTG | ATTTGATTAAACCTGCTGACTG |
SAN_0595 | -3.7 | -2.4 | AAGATAGTGCTCTCCTTCAAAC | CCCTTTCCCAAGATATTTCTCC |
SAN_0596 | -1.6 | -2.4 | GCAGGGATTATTGTTGAGC | GAAGTCTCTTGTGCGGATTTC |
SAN_1755 | -6.0 | -3.7 | GCAGATGAGGTAGAACAGTGG | GTGCGGTAATGTGACCTTTATC |
SAN_2186 | -3.7 | -3.2 | AATATAAGTTAGGTGCCGTATCTG | CGTGTTGTTAGTAGGTGTCTC |
SAN_1255 | -3.2 | -2.7 | AAACCCAAATCCTCACATTATTG | TCCCATCTTTACATTGACTTCG |
SAN_0156 | -1.7 | -2.6 | ATATAAAGGTTCTGCCAAAGTC | TGTATAAGTTGCTAAGTTCTCATC |
SAN_0157 | -2.8 | -2.9 | TTGGACATCATCTAATGGCTTAC | ATATGGCGGTCAATAGGAAATG |
SAN_0597 | -2.1 | -2.0 | GGCGGGATTGAGAGGTAAAC | TAACGGACATCATAACTAACTGC |
SAN_0933 | +1.7 | +2.5 | CAGAAATAAGGCATCAACTACC | AAAGAATAGGCATTATCAATACG |