Table 2 Phage KS10 putative genes and homologues
From: Genomic sequence and activity of KS10, a transposable phage of the Burkholderia cepacia complex
GP | Coding | Str. | Possible RBS* & Start Codon | AA | Putative Function | Alignment Region | % Identity | Score | % GC | Sig. matches to proteins in NCBI's GenBank |
|---|---|---|---|---|---|---|---|---|---|---|
1 | 205–1437 | - | AGGCGcgtaaATG | 410 | virion morphogenesis | 400(28–418)/248(16–263) | 30%/33% | 160/137 | 63.26 | Pseudomonas bacteriophage B3 gp33/BcepMu gp30 |
2 | 1438–1698 | - | AAAGGAGccaacaATG | 86 | hyp. protein | - | - | - | 61.69 | no sig. match |
3 | 1743–3278 | - | GGAAG//36 bp//cgtttATG | 511 | portal | 354(35–374) | 32% | 182 | 62.57 | BcepMu gp29 |
4 | 3268–4884 | - | AAGAGGcctgatccacgATG | 538 | terminase large subunit** | 520(4–521) | 55% | 569 | 58.94 | D3112 p26 |
5 | 4891–5388 | - | AAGGGttgacgcATG | 165 | terminase small subunit** | 164(1–164) | 37% | 161 | 59.24 | D3112 p24 |
6 | 5392–5682 | - | AGGGTATccgcgATG | 96 | hyp. protein | - | - | - | 59.45 | no sig. match |
7 | 5679–5903 | - | GGATAacgATG | 74 | dksA/traR C4-type zinc finger | 74(1–72) | 45% | 44.3 | 60.89 | Enterobateria phage WPhi gp82 |
8 | 5893–6186 | - | GAGGAtgcccgtcATG | 102 | hyp. protein | - | - | - | 65.99 | no sig. match |
9 | 6466–7137 | - | GTGGGcctcgcagcATG | 223 | endolysin | 186(20–203) | 54% | 188 | 65.03 | BcepMu gp22 |
10 | 7134–7535 | - | GGGTGccgccgtcgcgaATG | 133 | holin | 119(1–111)/116(8–113) | 36%/39% | 72.4/63.5 | 58.96 | Ralstonia solanacearum UW551***/BcepMu gp21 |
11 | 7650–8258 | - | GGGAAGcgcgaaATG | 202 | hyp. protein | 177(53–211) | 26% | 45.4 | 53.53 | Pseudomonas entomophila L48 |
12 | 8251–8784 | - | GGGAATAtgtaaagcATG | 177 | hyp. protein | - | - | - | 54.87 | no sig. match |
13 | 8839–9309 | - | AAGAGGccgaccATG | 156 | hyp. protein/phage repressor | 131(1–125)/137(2–127) | 39%/36% | 85.1/72.4 | 56.69 | E. coli B171/BcepMu gp17 |
14 | 9391–9582 | + | GGAGcaaatATG | 63 | DNA binding protein/conserved hyp. protein | 62(5–65)/59(1–57) | 33%/52% | 38.1/65.1 | 58.85 | BcepMu gp16/Ralstonia solanacearum UW551 |
15 | 9579–10631 | + | GGGGAGGtggATG | 350 | conserved hyp. protein | 141(49–186)/127(77–203) | 28%/28% | 38.1/44.7 | 64.71 | BcepMu gp10/Ralstonia solanacearum UW551 |
16 | 10671–12296 | + | AGGTGcgatATG | 541 | transposase | 539(24–559) | 36% | 336 | 65.19 | Ralstonia solanacearum UW551 |
17 | 12306–13298 | + | GAATAAGGAGtgaccATG | 330 | transposition protein | 309(7–311) | 42% | 222 | 64.15 | Ralstonia solanacearum UW551 |
18 | 13306–13494 | + | GTGAGGccatcATG | 62 | hyp. protein | - | - | - | 66.14 | no sig. match |
19 | 13491–13796 | + | GAGGTGAcggcATG | 101 | hyp. protein | - | - | - | 69.28 | no sig. match |
20 | 13859–14371 | + | TGGccgacgacagcATG | 170 | hyp. protein | 172(55–187) | 60% | 180 | 67.25 | Ralstonia solanacearum UW551 |
21 | 14368–14994 | + | AAGGAAcccatcATG | 208 | conserved protein | 200(9–210)/199(6–204) | 72%/55% | 210/205 | 63 | Ralstonia solanacearum UW551/BcepMu gp05 |
22 | 15005–15397 | + | GAGGGGctggccATG | 130 | hyp. protein | - | - | - | 66.16 | no sig. match |
23 | 15460–15732 | + | AGGAGAAAcaccctcATG | 90 | DNA binding protein Hu-beta | 90(1–90) | 57% | 108 | 64.47 | Bordetella pertussis Tohama I |
24 | 15809–16642 | + | GAGGAAccccaaaATG | 277 | hyp. protein | 117(51167)/58(344–401) | 53%/67% | 114 | 62.83 | Burkholderia thailandensis E264 gp38 |
25 | 16806–17126 | + | GGGGAGTGAcactgtgATG | 106 | hyp. protein | 111(9–119) | 37% | 55.1 | 64.49 | Burkholderia pseudomallei K96243 |
26 | 17128–17568 | + | GGAGGcccgctgacATG | 146 | modulation of host genes? | 129(9–135) | 30% | 58.5 | 64.17 | Escherichia coli B7A/Mu gp16 |
27 | 17565–17963 | + | GAAACGAccgcATG | 132 | middle operon regulator (Mor)**** | 106(14–118) | 31% | 52.8 | 64.91 | Pseudomonas entomophila L48 |
28 | 18120–19322 | + | AGAGAAccatTTG | 400 | protease | 330(10–322) | 33% | 147 | 65.59 | BcepMu gp32 |
29 | 18876–19322 | + | AGGAagccATG | 148 | scaffold | - | - | - | 67.11 | BcepMu gp33 |
30 | 19368–19724 | + | AGAGGATtcacATG | 118 | conserved hyp. protein | 77(41–117)/80(32–109) | 54%/42% | 72.4/44.3 | 71.71 | Bacteriophage B3 gp37/BcepMu gp35 |
31 | 19775–20722 | + | GGAGctatccATG | 315 | major head subunit | 311(4–307) | 47% | 243 | 64.87 | Bacteriophage B3 gp34 |
32 | 20797–21186 | + | AAGAGAGatcATG | 129 | hyp. protein | - | - | - | 66.92 | no sig. match |
33 | 21183–21686 | + | GAAGGGccgcaaATG | 167 | hyp. protein | - | - | - | 65.48 | no sig. match |
34 | 21683–22114 | + | GGATAGGTAcgggaaATG | 143 | virion morph. | 161(8–156) 121(7–124) | 31%/36% | 161/52.4 | 60.19 | Thermus aquaticus Y51MC23/Escherichia coli 53638 (MuG) |
35 | 22114–22716 | + | GGTGAGGATGAtgtgATG | 200 | phage-related conserved hyp. protein | 165(1–164) | 24% | 41.2 | 61.36 | Burkholderia thailandensis MSMB43 |
36 | 22700–22978 | + | ACGGG//25 bp//gatATG | 92 | hyp. protein | - | - | - | 64.52 | no sig. match |
37 | 23022–24500 | + | AAGGGAcattcgacATG | 492 | tail sheath protein | 472(12–481) | 34% | 188 | 63.15 | Prophage MuSo2 (Marinomonas sp.)/MuL (gp39) |
38 | 24546–24917 | + | AAGGGAGTGaaacATG | 123 | hyp. protein | - | - | - | 62.9 | no sig. match |
39 | 25000–25554 | + | TGAGATTcccaccATG | 184 | tail assembly chaperone | - | - | - | 62.88 | FluMu gp41** |
40 | 25603–28038 | + | GAGGAAGAgacgATG | 811 | tail tape measure (TP109 fam.) | 484(3–458) | 38% | 265 | 64.2 | Burkholderia vietnamiensis G4 |
41 | 28038–29408 | + | GGAGGAAcgaactgATG | 456 | DNA circulation protein | 455(3–412) | 27% | 150 | 65.43 | Polaromonas sp. JS666/MuN (gp47) |
42 | 29414–30571 | + | TGAcccctATG | 385 | tail protein | 352(96–337) | 33% | 153 | 63.04 | Polaromonas sp. JS666/MuP (gp44) |
43 | 30571–31092 | + | GGAGcaaactgATG | 173 | baseplate assembly | 60(36–188) | 30% | 63.5 | 61.88 | Escherichia. coli 0157:H7 str. EC4501/MuQ (gp45) |
44 | 31177–31758 | + | AGGccatcATG | 193 | tail protein | 99(25–112) | 47% | 70.9 | 64.6 | MuV (gp46) |
45 | 31755–32876 | + | GGAAAAcatcATG | 476 | tail protein | 306(24–325) | 36% | 144 | 65.86 | Pseudomonas entomophila L48/MuW (gp47) |
46 | 32879–33478 | + | AGGAGTgaccGTG | 173 | tail protein | 197(5–192) | 26% | 57 | 65.17 | Desulfovibrio vulgaris subsp. vulgaris str. Hildenborough |
47 | 33478–34455 | + | GGAcatcgactgATG | 325 | tail fiber protein | 229(310–534) | 42% | 140 | 62.47 | Burkholderia multivorans ATCC 17616 |
48 | 34463–36691 | + | TGAGGcacgcATG | 742 | ABC-type phosphate transport sys. | 459(40–493) | 46% | 364 | 60 | Pseudomonas stutzeri A1501 |
49 | 36711–37475 | + | GAGGTAcaaATG | 254 | hyp. protein | 270(1–248) | 28% | 81.3 | 59.48 | Klebsiella pneumoniae 342 KPK_4114 |