| FD | DTT | CsCl | MG |
---|---|---|---|---|
Purification steps | Filtration + DNase | DTT + Filtration + DNase | Filtration + DNase + CsCl centrifugation | Total metagenome of unpurified sample |
DNA recovered in (ng in sample 1, ng in sample 2) | 42, 44 | 29, 34 | 21, 19 | 636, 459 |
Sample throughputa | 15-20 | 15-20 | 6-8d | N/A |
Total duration of protocol (days)b | 1 | 1 | 2 | N/A |
Hands on time (hours)c | 6 | 6 | 10 | N/A |
Special equipment needed | No | No | Ultracentrifuge | N/A |
Intra-method reproducibility | High | High | Medium | N/A |
Biases against specific phage | Weak | Weak | Strong | N/A |
Removal efficiency of mouse DNA | High | High | Very high | N/A |
Removal efficiency of bacterial DNA | Very high | Very high | Very high | N/A |