Impacts of low coverage depths and post-mortem DNA damage on variant calling: a simulation study

BMC Genomics

Table 1 Impact of independent variables on the percent of reads mapped from read pools

Divergence	%GC	Read length	Damage	Coverage depth
Low	-0.1212*	-0.7032***	-6.4180***	-0.0005
High	-0.1068*	-0.8363***	-5.9020***	0.0008

Slope coefficients resulting from multiple variable regression analysis are shown applying best-fit linear models, considering as dependent variables the percent of reads mapped from a pool, while GC content, read length, damage level and coverage depth were treated as independent variables. Values of 1, 2 and 3 were assigned to variables no-, low- and high-damage, respectively. Values not significant at Pr < 0.05 unless otherwise indicated; * signifies 0.01 < Pr < =0.05; *** signifies Pr < =0.001.

ISSN: 1471-2164