Figure 11

Position of variants present in PR8 and PR8mut quasispecies in the HA, NP and NS1. The variants in HA (hemagglutinin), NP (nucleoprotein) and NS1 (non-structural protein 1) detected in the PR8 and PR8mut quasispecies were modeled with PyMol (Delano Scientific, http://www.pymol.org), using the HA from A/Puerto Rico/8/1934 (H1N1) (PDB code: 1RVX), the NP from A/Wilson-Smith/1933 (H1N1) (PDB code: 2IQH) and the effector domain of NS1 from A/Puerto Rico/8/1934 (H1N1) (PDB code: 3RVC). (A) Top (left) and lateral (right) view of the surface exposed amino acids of the HA trimer. The Cb, Ca, Sa and Sb antigenic sites are shown in green. The mutations that are present in both PR8 and PR8mut are shown in red or in magenta if they overlap with the antigenic sites. Mutations in PR8mut that are present at a frequency > 5% are shown in blue or in yellow when overlapping with the antigenic sites or in purple when overlapping with the fusion peptide (orange). The mature H3 amino acid numbering of the variants is provided in superscript. (B) Lateral view of the NP monomer with the N395S mutation present in PR8 shown in brown. (C) The effector domain of NS1 with the Q121H mutation in PR8mut shown in blue.