Figure 3

Differential binding of PU.1 and Spi-B within the genome. A. Distribution of PU.1 and Spi-B unique ChIP-seq peaks within the genome. Unique sites for these factors are frequently located beyond 10Kb of the transcription start site compared with the overall peak sets. B. De-novo motif analysis by MEME-ChIP of unique regions determined by DiffBind. The most frequently occurring motif for each sample is shown and compared with the most common motif found in all peaks for PU.1 and Spi-B. PU.1-unique peaks retained similar motifs to those seen in all PU.1 peaks, but just over half of these peaks also contained a POU2F2 motif. Spi-B unique peaks retained the ETS-IRF motif seen in all Spi-B peaks, with higher conservation of the IRF motif (dotted box). C. A unique PU.1 binding peak was observed at the site of mir155 D. Unique binding of PU.1 within the gene Nod2. E. Unique Spi-B binding in the 3′ end of Fgf23. F. Unique Spi-B binding in the promoter of Lat2.